The Aussie pop princess, 50, secretly signed up for the prestigious Sunday afternoon slot on the Pyramid Stage at the beginning of the year.
The Can’t Get You Out Of My Head singer, who has had seven UK No1 tunes during her three decade career, follows in the footsteps of previous performers Dolly Parton, 72, Lionel Richie, 69, and Barry Gibb, 71.
A festival insider said: “Kylie had to pull out of Glastonbury in 2005 just a month before she was due to appear so this performance will be a very emotional and poignant moment for her.
“She jumped at the opportunity to appear next year and it is the perfect way to celebrate her career.”
Speaking to The Sun in July about the possibility of joining the bill, she said: “When I was supposed to do it I think I would have been the first solo female to headline in however many years it was, so I was really proud of that at that time. Obviously it didn’t happen.
“So yes, it would be amazing and very emotional to be standing there and doing what I didn’t get to do all those years ago, for sure.”
Kylie eventually performed at Worthy Farm when she joined Scissor Sisters on stage in 2010 but she has not appeared at the festival as a solo artist.
When asked in July who would fill the slot, organiser Emily Eavis, 39, said: “I can’t tell you, but I’ll give you a clue: it’s a female. She is not British, but I can’t give you any more clues because it will get me into trouble.”