The popular star is best known for playing Alice Tinker in the BBC's The Vicar of Dibley.
Dawn French and Hugh Grant led the tributes to actress.
The British star was best known for her roles in The Vicar Of Dibley and Notting Hill.
A statement from her agency said Emma died from natural causes and that she would be “greatly missed”.
It said: “Emma created a wealth of characters and an immense body of work. She brought laughter and joy to many.”
Hugh, who starred alongside Emma in Notting Hill, said this afternoon: “Emma Chambers was a hilarious and very warm person and of course a brilliant actress. Very sad news.”
Dawn, who worked alongside Emma for years on The Vicar Of Dibley, tweeted: “I was regularly humped like this by the unique & beautiful spark that was Emma Chambers. I never minded. I loved her. A lot .”
Sad news just in... Emma Chambers has sadly passed away at the age of 53. Lovely lady, brilliant… https://t.co/zYLbtuvyrO— Dawn French (@DawnFrenchUK) February 24, 2018
Emma Chambers was a hilarious and very warm person and of course a brilliant actress. Very sad news.— Hugh Grant (@HackedOffHugh) February 24, 2018
Richard Curtis' wife Emma Freud also paid tribute to Emma and tweeted: “Our beautiful friend Emma Chambers has died at the age of 53.
“We're very very sad. She was a great, great comedy performer, and a truly fine actress.
“And a tender, sweet, funny, unusual, loving human being.”
Sharing a video of Emma as Alice Tinker in The Vicar Of Dibley, Emma added: “How could you not love this girl. Emma Chambers... thank you for the brilliance.”
Emma's Notting Hill co-star James Dreyfus added: “RIP the wonderful and talented Emma Chambers. Unique & unspeakably funny. Too young. Thoughts with her family. X”
Film producer Richard Shotcroft, said: “RIP the wonderful Emma Chambers - best known for The Vicar of Dibley but also stole every scene in Notting Hill. Only 53.”
Jeremy Clarkson paid tribute to Emma and told his followers he knew her before she made it big.
He told his Twitter followers: “I'm sad about Emma Chambers. Knew her when she was a kid in Doncaster. She was very funny.”
I'm sad about Emma Chambers. Knew her when she was a kid in Doncaster. She was very funny.— Jeremy Clarkson (@JeremyClarkson) February 24, 2018
Rylan Clark-Neal told his fans: “Gutting news about Emma Chambers. Really really sad. What an actress. Grew up with Alice what an absolute joy to watch. God bless xxx.”
Jodie Marsh also sent her condolences to Emma's family.
She tweeted: “Such terribly sad news about Emma Chambers. Notting Hill is one of my favourite films of all time and she is so stunningly superb in it. That film makes me laugh & cry, as does her performance in it. RIP Emma.”
Emma is survived by her husband, fellow actor Ian Dunn.
Emma captured international attention for her secondary role in the 1999 Hugh Grant and Julia Roberts comedy Notting Hill.
In the movie, she plays Honey, Hugh Grant's little sister and the romantic interest of Rhys Ifans' character Spike.