She has accused the organiser of the Grammys of lying about her reasons for withdrawing from performing at the ceremony.
The singer posted several tweets explaining her reasons for not attending the show after Grammys producer Ken Ehrlich said her absence was down to not being able to organise her set in time.
Grande denied this, saying she could "pull together a performance overnight", and instead said Ehrlich had "stifled" her creativity.
In her first post, the singer said: "I've kept my mouth shut but now you're lying about me. I can pull together a performance over night and you know that, Ken. It was when my creativity & self expression was stifled by you, that I decided not to attend. I hope the show is exactly what you want it to be and more."
Grande has been featured on billboards promoting the show, which takes place in Los Angeles on Sunday.
In her tweets, she accused organisers of "playing games".
"I offered 3 different songs," she said. "It's about collaboration. It's about feeling supported. It's about art and honesty. Not politics. Not doing favors or playing games. It's just a game y'all. And i'm sorry but that's not what music is to me."
She then joked that she had "passed a Grammy's bus with my face on it" as she was typing the tweets, before saying she was "still grateful for the acknowledgement this year".
The row comes as Grande releases her much anticipated fifth album, Thank U, Next.
The singer had received two Grammy nominations, for best pop vocal album for her last record, Sweetener - written following the Manchester Arena terror attack at her show there in May 2017 - and best pop solo performance, for God Is A Woman.
Ehrlich, who had said Grande "felt it was too late… to pull something together" for the ceremony, is yet to respond to her comments.
Artists who will perform at the ceremony include Cardi B, Dolly Parton, Red Hot Chili Peppers, Diana Ross, Camila Cabello, Brandi Carlile, Post Malone and British star Dua Lipa.
Lady Gaga is also performing, meaning she will miss out on the BAFTAs, which also take place on Sunday, with A Star Is Born co-star and director Bradley Cooper.
© Sky News 2019